国产精品香港三级国产av-波多野结衣av高清中文字幕-亚洲日韩欧美一区久久久久我-美女扒开屁股让男人桶

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>人正常細(xì)胞>CRL-9444MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

簡(jiǎn)要描述:MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞
ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞,細(xì)胞庫(kù)管理規(guī)范,提供 培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9444
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2024-11-16
  • 訪  問(wèn)  量:1940

產(chǎn)品分類

Product Category

相關(guān)文章

Related Articles

詳細(xì)介紹

MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

ATCC® Number:CRL-9444™    Price:$329.00
Designations:MeT-5A

Depositors:The United States of America

Biosafety Level:2 [Cells contain polyomavirus DNA sequences ]

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Tissue: mesothelium
Cell Type: epithelialvirus transformed


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MeT-5A細(xì)胞, 人類肋膜間皮細(xì)胞

Tumorigenic:No

DNA Profile (STR):Amelogenin: X,Y

CSF1PO: 10,12

D13S317: 11,13

D16S539: 12

D5S818: 12

D7S820: 10

THO1: 6,9.3

TPOX: 8

vWA: 15,18



Comments:Mesothelial cells were isolated from pleural fluids obtained from non-cancerous individuals.

The cells were transfected with the pRSV-T plasmid (an SV40 ori- construct containing the SV40 early region and the Rous sarcoma virus long terminal repeat) and cloned.

The cells can be used to screen chemical and biological agents for activity as growth factor, carcinogens, mutagens, etc.

The cells stain positively for vimentin, keratins and SV40 T antigen.



Propagation:ATCC complete growth medium: Medium199 with Earle's BSS, .75 mM L-glutamine and 1.25 g/L sodium bicarbonate supplemented with with 3.3 nM epidermal growth factor (EGF), 400 nM hydrocortisone, 870 nM insulin, 20 mM HEPES, trace elements (see above reference) and 10% fetal bovine serum.
Temperature: 37.0°C


Subculturing:Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. To remove trypsin-EDTA solution, transfer cell suspension to centrifuge tube and spin at approximay 125 xg for 5 to 10 minutes.Discard supernatant and resuspend cells in fresh growth medium. Add appropriate aliquots of cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.


Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:4 is recommended
Medium Renewal: Every 2 to 3 days


Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase


Related Products:recommended serum:ATCC 30-2020

References:21937: Reddel RR, et al. Immortalized human bronchial epitherial mesothelial cell lines. US Patent 4,885,238 dated Dec 5 1989
23292: Lechner JF, et al. Asbestos-associated chromosomal changes in human mesothelial cells. Proc. Natl. Acad. Sci. USA 82: 3884-3888, 1985. PubMed: 2987952

























產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:xiangfbio@163.com

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2024 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 亚洲国产一二三精品无码| 特级毛片a级毛片免费播放| 欧美丰满熟妇aaaaa片| 亚洲人交乣女bbw| 亚洲中文字幕在线第六区| 女人大荫蒂毛茸茸视频| 日出水了特别黄的视频| 与子敌伦刺激对白播放的优点| 久久精品国产首页027007| 午夜亚洲福利在线老司机| 亚洲国产日韩欧美一区二区三区| 精品亚洲aⅴ在线观看| 午夜福利av无码一区二区| 久久午夜无码鲁丝片直播午夜精品 | 国产无遮挡又黄又爽高潮| 午夜dv内射一区区| 国产丰满老熟女重口对白| 精品少妇人妻av免费久久久| 日韩乱码人妻无码中文字幕久久 | 玩弄人妻少妇500系列视频| 少妇精品无码一区二区三区| 天天摸天天摸色综合舒服网| 免费一本色道久久一区| 老师在办公室被躁在线观看| 粉嫩大学生无套内射无码卡视频 | 欧美性猛交xxxx免费看蜜桃 | 超碰最新上传| 黄色视频免费在线观看 | 97久人人做人人妻人人玩精品| 第一次挺进莹莹的身体里视频| 久久天天躁狠狠躁夜夜av浪潮| 国产精品无码一区二区在线| 麻豆一区二区三区蜜桃免费| 国产精品久久久国产盗摄| 456亚洲影院| 97久久天天综合色天天综合色hd| 久久99精品久久水蜜桃| 无遮挡边吃摸边吃奶边做| 欧美人妻少妇精品久久黑人| 亚洲av无码乱码在线观看代蜜桃| 精品多毛少妇人妻av免费久久|